环境：
VS2010 活动解决方案平台x64
WIN 8.1
Opencv 2.4.3
Matlab 2012a
1、 首先保证vs2010能正确调用opencv函数，
2、 Matlab中选择编译器，操作如下：
打开matlab2012，输入mex –setup，出现如下提示：
Welcome to mex -setup. This utility will help you set up
a default compiler. For a list of supported compilers, see
http://www.mathworks.com/support/compilers/R2012a/win32.html
Please choose your compiler for building MEX-files:
Would you like mex to locate installed compilers [y]/n?
输入y，出现如下提示：
Select a compiler:
[1] Lcc-win32 C2.4.1 in C:\PROGRA~1\MATLAB\R2012a\sys\lcc
[2] Microsoft Visual C++ 2010 in C:\Program Files\Microsoft Visual Studio 2010
[0] None
Compiler:
输入，出现如下提示：
Please verify your choices:
Compiler: Microsoft Visual C++ 2010
Location: C:\Program Files\Microsoft Visual Studio 2010
Are these correct [y]/n?
输入y
出现如下提示，表明成功：
***************************************************************************
Warning: MEX-files generated using Microsoft Visual C++ 2010 require
that Microsoft Visual Studio 2010 run-time libraries be
available on the computer they are run on.
If you plan to redistribute your MEX-files to other MATLAB
users, be sure that they have the run-time libraries.
***************************************************************************
Trying to update options file: C:\Documents and Settings\zhangjing\Application Data\MathWorks\MATLAB\R2012a\mexopts.bat
From template: C:\PROGRA~1\MATLAB\R2012a\bin\win32\mexopts\msvc100opts.bat
Done . . .
**************************************************************************
Warning: The MATLAB C and Fortran API has changed to support MATLAB
variables with more than 2^32-1 elements. In the near future
you will be required to update your code to utilize the new
API. You can find more information about this at:
http://www.mathworks.com/help/techdoc/matlab_external/bsflnue-1.html
Building with the -largeArrayDims option enables the new API.
**************************************************************************
3、 配置一些目录
在matlab中，输入mex –v
下面会有显示：
-> Default options filename found in C:\Documents and Settings\zhangjing\Application Data\MathWorks\MATLAB\R2012a
----------------------------
-> Options file = C:\Documents and Settings\zhangjing\Application Data\MathWorks\MATLAB\R2012a\mexopts.bat
MATLAB = C:\PROGRA~1\MATLAB\R2012A
-> COMPILER = cl
-> Compiler flags:
COMPFLAGS = /c /GR /W3 /EHs /D_CRT_SECURE_NO_DEPRECATE /D_SCL_SECURE_NO_DEPRECATE /D_SECURE_SCL=0 /DMATLAB_MEX_FILE /nologo /MD
OPTIMFLAGS = /O2 /Oy- /DNDEBUG
DEBUGFLAGS = /Z7
arguments =
Name switch = /Fo
。。。。。。。。。（此处省略一些）
选中上面Options file=后面的部分，右键->Open Selection，打开mexopts.bat
找到下面部分，增加红色的部分
set MATLAB=%MATLAB%
set VSINSTALLDIR=C:\Program Files\Microsoft Visual Studio 2010
set VCINSTALLDIR=%VSINSTALLDIR%\VC
rem In this case, LINKERDIR is being used to specify the location of the SDK
set LINKERDIR=C:\Program Files\Microsoft SDKs\Windows\v7.0A\
set PATH=%VCINSTALLDIR%\bin;%VCINSTALLDIR%\VCPackages;%VSINSTALLDIR%\Common7\IDE;%VSINSTALLDIR%\Common7\Tools;%LINKERDIR%\bin;%MATLAB_BIN%;%PATH%
set INCLUDE=%VCINSTALLDIR%\INCLUDE;%VCINSTALLDIR%\ATLMFC\INCLUDE;%LINKERDIR%\include;%INCLUDE%;D:\opencv\include;D:\opencv\include\opencv;D:\opencv\include\opencv2
PS:本人导入的包含如下
;D:\Program Files\opencv\modules\world\include;D:\Program Files\opencv\modules\core\include;D:\Program Files\opencv\modules\videostab\include;D:\Program Files\opencv\modules\video\include;D:\Program Files\opencv\modules\ts\include;D:\Program Files\opencv\modules\stitching\include;D:\Program Files\opencv\modules\photo\include;D:\Program Files\opencv\modules\ocl\include;D:\Program Files\opencv\modules\objdetect\include;D:\Program;Files\opencv\modules\nonfree\include;D:\Program Files\opencv\include\opencv2;D:\Program Files\opencv\include\opencv;D:\Program Files\opencv\modules\ml\include;D:\Program Files\opencv\modules\legacy\include;D:\Program Files\opencv\modules\imgproc\include;D:\Program Files\opencv\modules\highgui\include;D:\Program Files\opencv\modules\gpu\include;D:\Program Files\opencv\modules\flann\include;D:\Program Files\opencv\modules\features2d\include;D:\Program Files\opencv\include;D:\Program Files\opencv\modules\contrib\include;D:\Program Files\opencv\modules\calib3d\include;D:\Program Files\opencv\modules\androidcamera\include
set LIB=%VCINSTALLDIR%\LIB;%VCINSTALLDIR%\ATLMFC\LIB;%LINKERDIR%\lib;%MATLAB%\extern\lib\win32;%LIB%;D:\opencv\build\x86\vc10\lib
PS:本人导入的包含如下
;D:\Program Files\opencv\build\x64\vc10\lib
set MW_TARGET_ARCH=win32
rem ********************************************************************
rem Compiler parameters
rem ********************************************************************
set COMPILER=cl
set COMPFLAGS=/c /GR /W3 /EHs /D_CRT_SECURE_NO_DEPRECATE /D_SCL_SECURE_NO_DEPRECATE /D_SECURE_SCL=0 /DMATLAB_MEX_FILE /nologo /MD
set OPTIMFLAGS=/O2 /Oy- /DNDEBUG
set DEBUGFLAGS=/Z7
set NAME_OBJECT=/Fo
rem ********************************************************************
rem Linker parameters
rem ********************************************************************
set LIBLOC=%MATLAB%\extern\lib\win32\microsoft
set LINKER=link
set LINKFLAGS=/dll /export:%ENTRYPOINT%
/LIBPATH:"%LIBLOC%" opencv_core231d.lib opencv_highgui231d.lib opencv_video231d.lib opencv_ml231d.lib opencv_legacy231d.lib opencv_imgproc231d.lib opencv_flann231d.lib opencv_features2d231d.lib opencv_calib3d231d.lib opencv_objdetect231d.lib opencv_contrib231d.lib libmx.lib libmex.lib libmat.lib /MACHINE:X86 kernel32.lib user32.lib gdi32.lib winspool.lib comdlg32.lib advapi32.lib shell32.lib ole32.lib oleaut32.lib uuid.lib odbc32.lib odbccp32.lib /nologo /manifest /incremental:NO /implib:"%LIB_NAME%.x" /MAP:"%OUTDIR%%MEX_NAME%%MEX_EXT%.map"
set LINKOPTIMFLAGS=
（注意上面include lib处要加分号，LINKFLAGS处各个lib之前要回空格）
PS:本人导入的lib如下：
opencv_calib3d243d.lib opencv_contrib243d.lib opencv_core243d.lib opencv_features2d243d.lib opencv_flann243d.lib opencv_gpu243d.lib opencv_haartraining_engined.lib opencv_highgui243d.lib opencv_imgproc243d.lib opencv_legacy243d.lib opencv_ml243d.lib opencv_nonfree243d.lib opencv_objdetect243d.lib opencv_photo243d.lib opencv_stitching243d.lib opencv_ts243d.lib opencv_video243d.lib opencv_videostab243d.lib
在matlab当前目录下建立cpp文件（简单方法，txt改后缀为cpp），vs打开敲入代码，代码需要是Matlab的C语言接口风格，函数内部调用OpenCV函数演示功能，简化后的代码：程序命名为useOpenCV.cpp保存。
#include"mex.h"
#include "cv.h"
#include "highgui.h"
using namespacecv;
voidmexFunction (int nlhs, mxArray *plhs[], // 输出参数个数，及输出参数数组
int nrhs, const mxArray *prhs[]) // 输入参数个数，及输入参数数组
{
char name[256];
int buflens =mxGetNumberOfElements(prhs[0]);
mxGetString(prhs[0], name, buflens+1);
if(!mxIsChar(prhs[0]))
{
mexErrMsgTxt("First parameter must be string/n");
}
mexPrintf(name);
IplImage * img = cvLoadImage(name, 1);
if(img->imageData == NULL)
{
mexErrMsgTxt("Error in image/n");
}
cvNamedWindow("1",1);
//imshow("1",mat);
cvShowImage("1",img);
cvWaitKey(0);
return;
}
5 在matlab里输入 mexuseOpenCV.cpp编译。
6 在matlab里输入命令调用编译好的文件：useOpenCV(‘lena.bmp’);即可调用。注意，lena.bmp图片放在当前文件下。